AP22919PU-N 4E-BP2 (99-120) antibody

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50 µg / $425.00
Delivery Time: 5-7 working days*
(*) valid for US customers only

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Rabbit anti Bovine, Canine, Human, Mouse, Porcine, Rat 4E-BP2

Product Description for 4E-BP2

Rabbit anti Bovine, Canine, Human, Mouse, Porcine, Rat 4E-BP2.
Properties: (99-120)
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for 4E-BP2

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms EIF4EBP2, Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2
Presentation Purified
Reactivity Bov, Can, Hu, Ms, Por, Rt
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q13542
Immunogen:
EIF4EBP2 antibody was raised against human PHAS-II synthetic peptide (residues 99-120) was synthesized and the peptide coupled to KLH.
GeneID:
1979
Remarks:
This sequence is 96% homologous to mouse PHAS-11 over these residues
Property 99-120
Application Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Western Blot: 4 µg/ml.
Background PHAS-II, also known as eIF4E-binding protein II (eIF4E-BPII), is a member of a family of proteins which regulate initiation. PHAS-I and -II were found to have overlapping but different patterns of expression in tissues. PHAS-II shows the highest expression in liver and kidney where very little PHAS-I is found. The PHAS proteins regulate translation initiation by binding to the inhibitory protein eIF- 4E and blocking translation by preventing access of eIF-4G to the 5' cap of the mRNA. Both PHAS proteins are phosphorylated in response to insulin or growth factors such as EGF, PDGF and IGF-1. Phosphorylation in the appropriate site(s) promotes dissociation of PHAS/eIF-4E complexes which allows eIF-4E to bind eIF-4G(p220), thereby increasing the amount of eIF-4F complex and the rate of translation initiation. Regulation of the two protein differ because PHAS-II, unlike PHAS-I is readily phosphorylated by PKA in vitro. However increasing cAMP in cells promotes dephosphorylation of both PHAS-I and PHAS-II. Pharmacological and genetic evidence indicates that the mTOR/p70S6K pathway is involved in the control of PHAS-I and -II suggesting that these proteins may be mediators of the effects of this pathway on protein synthesis and cell proliferation.
Concentration 1 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Immunoaffinity Chromatography
Buffer System:
BBS, pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (expected):
Bovine, Canine, Porcine, Mouse and Rat.
Species reactivity (tested):
Human.
Specificity
Specificity:
Detects 17 and 20 kD proteins, corresponding to the apparent molecular mass of PHAS-II and its phosphorylated state on SDS-PAGE immunoblots.
Gene ID 1979

Accessory Products

Proteins and/or Positive Controls

Proteins for 4E-BP2 (2 products)

Catalog No. Species Pres. Purity   Source  

4E-BP2 (1-120, His-tag)

Recombinant human EIF4EBP2, 1-120 aa, His-tagged Human Purified > 85 % E. coli
0.5 mg / $820.00
  OriGene Technologies GmbH

4E-BP2 (1-120, His-tag)

Recombinant human EIF4EBP2, 1-120 aa, His-tagged Human Purified > 85 % E. coli
0.1 mg / $320.00
  OriGene Technologies GmbH
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